|
KMID : 0380620180500060653
|
|
Korean Journal of Food Science and Technology
2018 Volume.50 No. 6 p.653 ~ p.659
|
|
|
The effects of antioxidant and anti-aging treatment of UVB-irradiated human HaCaT keratinocytes with ethanol extracts of colored rice varieties
|
|
Choi Eun-Young
Lee Jin-Tae
|
|
|
Abstract
|
|
|
|
In this study, we investigated the antioxidant activities and anti-aging efficacy in terms of suppression of matrix metalloproteinases (MMPs) in UVB-irradiated HaCaT cells by adding the ethanol extracts of Josaengheogchal (JE) and Shintoheug rice (SRE). In the electron-donating ability and ABTS radical-scavenging assays, we observed that both JE and SRE had scavenging activities and in a collagenase inhibition assay, both extracts showed inhibition effects of over 73% at 1,000 µg/mL concentration. The expression of MMP-1 and -3, when the extracts were treated with UVB 50 mJ/cm2, irradiated human HaCaT keratinocytes, was analyzed by western blotting and reverse-transcription polymerase chain reaction (RT-PCR). The results showed that MMP-1 and -3 proteins and mRNAs were downregulated in a concentration-dependent manner in response to both extracts. Therefore, we expect that these compounds have a potential for the use as functional ingredients with anti-aging effects in the cosmetic and food industries.
|
|
|
KEYWORD
|
|
|
anti-aging, colored rice, human HaCaT keratinocytes, matrix metallopreinases
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|